LOCUS       pLV-[hsa-miR-519a-1]        9159 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7080..7940
                     /label=Amp(R)
     promoter        8141..8681
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5685..5919
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6494..6949)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5991..6121
                     /label=SV40 pA
     misc_feature    6122..6335
                     /label=SV40 ori
     misc_feature    5006..5602
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3624
                     /label=intron
     CDS             3671..4996
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3414
                     /label=hsa-mir-519a-1 precursor
BASE COUNT     2190 a      2357 c      2477 g      2135 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg gtccttttag 
     3121 gggtttatgt tctggattct agaaaacaca caaaccggac aaataaatgc atctttattt 
     3181 ttgtgtctat tttaacctcg tcaaggaaga ttccaacaaa aaatccacag tgccggagca 
     3241 agaagacctc aggctgtgac actctagagg gaagcgcttt ctgttgtctg aaagaaagga 
     3301 aagtgcatcc ttttagagtg ttactgtttg agaaaagcaa cattgaagct gatgctgatc 
     3361 ttggtaataa atttgcagcg aatgcttata atcagacttg gatgatgttg ggctgctagc 
     3421 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt 
     3481 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc 
     3541 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt 
     3601 caaagttttt ttcttccatt tcaggtgtcg tgaaaactac ccctctagag tcgagctacc 
     3661 ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat 
     3721 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga 
     3781 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg 
     3841 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta 
     3901 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa 
     3961 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc 
     4021 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga 
     4081 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc 
     4141 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca 
     4201 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc 
     4261 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga 
     4321 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagga 
     4381 cccaccggtc gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 
     4441 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 
     4501 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 
     4561 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 
     4621 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 
     4681 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 
     4741 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 
     4801 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 
     4861 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 
     4921 cttcaccgtc accgccgacg tcgagtgccc gaaggaccgc gcgacctggt gcatgacccg 
     4981 caagcccggt gcctgagcgg ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa 
     5041 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 
     5101 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 
     5161 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 
     5221 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 
     5281 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 
     5341 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 
     5401 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 
     5461 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 
     5521 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 
     5581 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca 
     5641 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 
     5701 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 
     5761 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 
     5821 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 
     5881 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 
     5941 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 
     6001 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     6061 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
     6121 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 
     6181 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 
     6241 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc 
     6301 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt 
     6361 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 
     6421 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 
     6481 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 
     6541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 
     6601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 
     6661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 
     6721 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 
     6781 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 
     6841 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 
     6901 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt 
     6961 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     7021 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 
     7081 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 
     7141 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 
     7201 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 
     7261 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 
     7321 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 
     7381 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 
     7441 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 
     7501 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 
     7561 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 
     7621 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 
     7681 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 
     7741 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 
     7801 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 
     7861 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 
     7921 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 
     7981 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 
     8041 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 
     8101 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 
     8161 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 
     8221 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 
     8281 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 
     8341 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 
     8401 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 
     8461 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 
     8521 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 
     8581 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 
     8641 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 
     8701 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 
     8761 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 
     8821 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 
     8881 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
     8941 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
     9001 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
     9061 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg 
     9121 caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
